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Karthick Raja Namasivayam, S.
- Evaluation of Lignocellulosic Agro Wastes for the Enhanced Production of Extracellular Cellulase and Xylanase by Trichoderma harzianum
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1 Department of Biotechnology, Sathyabama University, Chennai 600 119, T.N., IN
2 Department of Chemical Engineering, Sathyabama University, Chennai 600 119, T.N., IN
1 Department of Biotechnology, Sathyabama University, Chennai 600 119, T.N., IN
2 Department of Chemical Engineering, Sathyabama University, Chennai 600 119, T.N., IN
Source
Nature Environment and Pollution Technology, Vol 14, No 1 (2015), Pagination: 47-52Abstract
In the present study, various lignocellulotic agro-wastes paddy straw, coir pith, sugarcane bagassae and dried leaf litter were evaluated for cellulase and xylanase enzyme production adopting solid state fermentation with different moisture levels ranging from 0 to 50% by soil isolate of Trichoderma harzianum. The crude enzyme obtained was partially purified by ammonium sulphate precipitation and DEAE cellulose column chromatography. All the wastes supported growth and enzyme production. Maximum cellulase and xylanase production was recorded in paddy straw followed by sugarcane bagassae with 25% moisture content. Both the enzymes retained maximum activity at 60°C and pH 8.0. The partially purified enzyme by ammonium sulphate precipitation at 60% saturation followed by DEAE cellulose column chromatography yields pure xylanase with the molecular weight of 56 and 61 KD.Keywords
Agro Wastes, Trichoderma harzianum, Cellulase, Xylanase, Enzyme Activity.- Biocompatible Formulation of Potential Fungal Biopesticide Nomuraea rileyi (f.) Samson for the Improved Post Treatment Persistence and Biocontrol Potential
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Authors
Affiliations
1 Department of Biotechnology, Sathyabama University, Chennai, Tamil Nadu, IN
1 Department of Biotechnology, Sathyabama University, Chennai, Tamil Nadu, IN